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KMID : 0383820090660040274
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Tuberculosis and Respiratory Diseases
2009 Volume.66 No. 4 p.274 ~ p.279
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Expression of COX-2 and IDO by Uteroglobin Transduction in NSCLC Cell Lines
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Park Gun-Min
Han Sung-Koo
Shim Young-Soo
Yim Jae-Joon
Lee Sang-Min
Yoo Chul-Gyu
Kim Young-Whan
Yang Seok-Chul
Lee Choon-Taek
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Abstract
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Background: Uteroglobin (UG) is a secretary protein that has strong immunomodulatory properties, and which
is synthesized in most epithelia including lung tissue. Overexpression of UG is associated with decreased expression
of cyclooxygenase (COX)-2 and suppression of cancer cell growth. Indoleamine 2,3-dioxygenase (IDO) catalyzes
tryptophan along the kynurenine pathway, and both the reduction in local tryptophan and the production of tryptophan metabolites contribute to the immunosuppressive effects of IDO.
Methods: In this study, we investigated the pattern of expression of COX-2 and IDO, and the effect of UG transduction in the expression of COX-2 and IDO in several non-small cell lung cancer cell lines, especially A549.
Results: Both COX-2 and IDO were constitutionally expressed in A549 and H460 cells, and was reduced by UG
transduction. In A549 cells, the slightly increased expression of COX-2 and IDO with the instillation of interferon-gamma (IFN-γ) was reduced by UG transduction. However, the reduced expression of COX-2 and IDO by UG transduction was not increased with IFN-γ instillation in A549 cells. In both the A549 COX-2 sense and the A549 COX-2 anti-sense small interfering RNA (siRNA)-transfected cells, IDO was expressed; expression was reduced by UG transduction, irrespective of the expression of COX-2.
Conclusion: The results suggest that the anti-proliferative function of UG may be associated with the immune
tolerance pathway of IDO, which is independent of the COX-2 pathway.
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KEYWORD
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Uteroglobin, Cyclooxygenase 2, Indoleamine 2, 3-dioxygenase, Interferon-gamma, Immune tolerance
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